Journal: Communications Biology
Article Title: Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study
doi: 10.1038/s42003-023-05378-w
Figure Lengend Snippet: A mounted light-emitting diode (LED) (M810L3 or M730L5, Thorlabs, Newport, NJ, USA) with a wavelength of 810 nm and 25 nm bandwidth, or 730 nm and 40 nm bandwidth, is used as an extended source (S1) of 1 mm 2 with 61.8 μW mm −2 , or 13.1 μW mm −2 irradiance, respectively. A first pair of air doublet lenses (L1) is used (AC254-030-B-ML and AC254-150-B-ML, Thorlabs, Newport, NJ, USA) to image S1 onto a first diaphragm (Ir1). A pair of silver mirrors (M1 and M2) (PF10-03-P01, Thorlabs, Newport, NJ, USA) are used to steer the light into the microscope. An air doublet (L2) (AC254-100-B-ML, Thorlabs, Newport, NJ, USA) is used to image Ir1 onto the back focal plane of both objectives (Obj.1 and Obj.2) (UPLSAPO30XSIR, Olympus, Japan) by the intermediate of an air doublet (L3 and L4) (AC254-150-B-ML, Thorlabs, Newport, NJ, USA), respectively. A second iris (Ir2) is imaged onto the sample and M4, a silicon mirror (monocrystalline silicon wafer, Nanomaterials Development Experts Store, China) and is conjugated to a third iris (Ir3) by the intermediary of a non-polarizer-beam-splitter (NPBS), itself conjugated to a cMOS camera (Q-2HFW, Adimec, Netherland) by the intermediary of a pair of air doublet lens es(L5) (AC254-060-B-ML and AC254-200-B-ML, Thorlabs, Newport, NJ, USA). A steering mirror (M3) (PFE10-P01, Thorlabs, Newport, NJ, USA) and a manual translation stage (XY Trans) are used for alignment and fine positioning of Obj.2; standing vertically. A piezoelectric (PZT) (PK25LA2P2, Thorlabs, Newport, NJ, USA) and linear stage (Delay stage) (X-LSQ075A, Zaber, Canada) are used to introduce a fast or fixed phase shift between interferometric fields, respectively. A flat mirror (M5) (CCM1-P01/M, Thorlabs, Newport, NJ, USA) is mounted in the turret (IX3-RFACA-1–3, Rapp Optoelectronic, Germany) of a commercial microscope (IX83, Olympus, Japan). A commercial stage top incubator (H201-K-FRAME, H201-MW-HOLDER, and OBJ-COLLAR-2532, Okolab, Italy) enables temperature and humidity control, and CO 2 , O 2 , and N 2 gas composition whilst hosting conventional glass-bottomed multiwell plates. A 2-dimensional translation stage (SCANplus IM 120 × 80, 2 mm, Marzhauser, Germany) enables scanning from well to well, as well as mosaicking, and the objective holder of the microscope, mounted on a linear stage (U-MCZ-1-2, Olympus, Japan) enables depth (Z) scanning.
Article Snippet: A first pair of air doublet lenses (L1) is used (AC254-030-B-ML and AC254-150-B-ML, Thorlabs, Newport, NJ, USA) to image S1 onto a first diaphragm (Ir1).
Techniques: Microscopy, Introduce, Control
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